Generation of αGFP-nanobodies suitable for super-resolution imaging of nuclear proteins

  • Niklas Bachmann University of Rotterdam

Abstract

The emergence of single molecule localization microscopy (SMLM) techniques made the imaging of cells at resolutions far beyond the diffraction barrier possible. However, the usual approach of tagging a protein of interest (PoI) with a primary antibody, and tagging this one with a fluorophore-carrying secondary antibody, introduces a significant displacement of the signal from the PoI. Here, the generation and application of an αGFP-nanobody is described which, through its reduced size and direct fluorophore labeling, leads to a much higher co-localization of signal and PoI and qualifies for dSTORM imaging of nuclear proteins.

How to Cite
BACHMANN, Niklas. Generation of αGFP-nanobodies suitable for super-resolution imaging of nuclear proteins. Student Undergraduate Research E-journal!, [S.l.], v. 4, nov. 2018. ISSN 2468-0443. Available at: <https://journals.library.tudelft.nl/index.php/sure/article/view/2833>. Date accessed: 14 dec. 2018. doi: https://doi.org/10.25609/sure.v4.2833.
Published
2018-11-09